Keep It Real – with Native Protein MS

Keep It Real – with Native Protein MS

Presented By: David Wong & Christian Klein

Speaker Biography:

Webinar: Keep It Real – with Native Protein MS

Webinar Abstract: We present a novel workflow for native MS, using a robust and sensitive LC/MS method using standard LC flow for native protein analysis. Most of the native MS analyses are using a nano-electrospray approach, which faces significant challenges in handling as well as throughput.
The workflow comprised the Agilent 1290 Infinity II LC, the AdvanceBio SEC column, the 6545XT AdvanceBio LC/Q-TOF, and MassHunter BioConfirm software. Examples from ligand-binding proteins, MAb’s as well as non-covalent complexes will be shown using standard-flow conditions, demonstrating charge-state distribution previously only observed with nano-spray.

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